Movie Gallery

Movie 1.
A T cell expressing CD3 ζ-YFP (green color) makes the immunological synapse with a B cell coated with the agonist peptide. The T cell moves away from the B cell but the cell-cell contact remains in form of a tether. (2.2MB)

Movie 2.
The T cell expresses CD4-YFP (green). B cells, loaded with the agonist peptide, are not visible but present in the field of view. They can be seen in one DIC frame, about 2/3 into the movie. The T cell approaches a B cell lying on the upper right and quickly clusters CD4 in the contact site. Then it migrates away but the contact remains in form of a tether connecting the cells. Note how the cytoplasm of the T cell flows back into the tether. The movie is shown overexposed to better visualize the tether. (1.6MB)

Movie 3.
Multiple synapses formed by a single T cell. This is a 3D rotation of a live T cell expressing CD4-YFP. This cell forms several immunological synapses with agonist peptide-loaded B cells (not visible). The contacts can be seen as the areas on the T cell surface enriched in CD4. (0.3MB)

Movie 4.
This is a 3D rotation of a live T cell-dendritic cell conjugate. T cell expresses CD3ζ-CFP in blue and CD4-YFP in green. DC fluoresces red. Note almost absolute recruitment of CD4 to the synapse area. (0.82MB)

Movies are by Tomasz Zal, Ph.D. and Anna Zal, M.Sc., Scripps 2000

Copyright Gascoigne Lab 2013