The Wyatt Laboratory

Selected Publications

Wyatt R, Kwong P, Desjardins E, Sweet R, Robinson J, Hendrickson W, Sodroski J. The antigenic structure of the human immunodeficiency virus gp120 envelope glycoprotein. Nature. 1998;393:705-11.

Kwong P, Wyatt R, Desjardins E, Sweet R, Robinson J, Sodroski J, Hendrickson W. Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 and a neutralizing human antibody. Nature. 1998;393:648-59.

Wyatt R, Sodroski J. The HIV-1 envelope glycoproteins: fusogens, antigens and immunogens.  Science. 1998;280:1884-88.

Pancera M, Wyatt R. Selective recognition of oligomeric HIV-1 primary isolate envelope glycoproteins by potently neutralizing ligands requires efficient precursor cleavage. Virology. 2005 Feb 5;332(1):145-56.

Pancera M, Lebowitz J, Schon A, Zhu P, Freire E, Kwong PD, Roux KH, Sodroski J, Wyatt R. Soluble mimetics of human immunodeficiency virus type 1 viral spikes produced by replacement of the native trimerization domain with a heterologous trimerization motif: characterization and ligand binding analysis. J Virol. 2005 Aug;79(15): 9954-69.

Huang CC, Tang M, Zhang MY, Majeed S, Montabana E, Stanfield RL, Dimitrov DS, Korber B, Sodroski J, Wilson IA, Wyatt R, Kwong PD. Structure of a V3-containing HIV-1 gp120 core. Science. 2005 Nov 11; 310(5750):1025-8

Li Y, Svehla K, Mathy NL, Voss G, Mascola JR, Wyatt R. Characterization of Antibody Responses Elicited by Human Immunodeficiency Virus Type 1 Primary Isolate Trimeric and Monomeric Envelope Glycoproteins in Selected Adjuvants. J Virol. 2006 Feb;80(3):1414-26.

Zhou T, Xu L, Dey B, Hessel A, Van Ryk D, Xiang S-H, Yang X, Zhang M-Y, Zwick M, Arthos J, Burton DR, Dimitrov DS, Sodroski J, Wyatt R, Nabel GJ, PD Kwong. Structural definition of a conserved neutralization epitope on HIV-1 gp120. Nature. 2007 Feb 15;445(7129):732-7.

Dey, B, Pancera M, Svehla K, Shu Y, Li Y, Xiang S-H, Vainshtein J, Sodroski J, Kwong PD, Mascola J, R Wyatt. Characterization of Human Immunodeficiency Virus Type 1 Monomeric and Trimeric gp120 Glycoproteins Stabilized in the CD4-bound State: Antigenicity, Biophysics and Immunogenicity. J Virol. 2007 Mar 14

Phogat S, Svehla K, Tang M, Spadaccini A, Muller J, Mascola J,     Berkower I,     Wyatt R, Analysis of the human immunodeficiency virus type 1 gp41 membrane proximal external region arrayed on hepatitis B surface antigen particles. Virology. 2007 Dec 21

Karlsson Hedestam GB, Fouchier RA, Phogat S, Burton DR, Sodroski J, Wyatt    RT.The challenges of eliciting neutralizing antibodies to HIV-1 and to influenza virus.Nat Rev Microbiol. 2008 Feb;6(2):143-55.

Forsell M, Dey B, Mörner A, Svehla K, O'Dell S, Högerkorp C, Voss G,      Thorstensson R, Shaw G, Mascola J, Karlsson Hedestam G, Wyatt RT. B Cell Recognition of the Conserved HIV-1 Co-Receptor Binding Site Is Altered by Endogenous Primate CD4. PloS Pathogens 2008 Oct 3;4(10)

Li Y, Svehla K, Louder MK, Wycuff D, Phogat S, Tang M, Migueles SA, Wu X,        Phogat A, Shaw GM, Connors M, Hoxie J, Mascola JR, Wyatt R. Analysis of the    Neutralization Specificities in Polyclonal Sera Derived from Human Immunodeficiency Virus Type-1 Infected Individuals. J Virol 2009; 83 (2):1045-59

N. A. Doria-Rose, R. M. Klein, M. M. Manion, S. O'Dell, A. Phogat, B. Chakrabarti, C. W. Hallahan, S. A. Migueles, J. Wrammert, R. Ahmed, M. Nason, R. T. Wyatt, J. R. Mascola, and M. Connors. Frequency and Phenotype of HIV Envelope-specific B Cells From Patients With Broadly Cross-Neutralizing        Antibodies. J Virol. 2009 Jan;83(1):188-99

Andreas Morner, Iyadh Douagi, Mattias N E Forsell, Christopher Sundling, Pia       Dosenovic, Sijy O'dell, Barna Dey, Peter D Kwong, Gerald Voss, Rigmor Thorstensson, John R Mascola, Richard T Wyatt, and Gunilla B Karlsson          Hedestam, HIV-1 Env-trimer immunization of macaques and impact of priming       with viral vector or stabilized core protein. J Virol. 2008; Jan;83(2):540-51

Scheid JF, Mouquet H, Feldhahn N, Walker BD, Pereyra F, Cutrell E, Seaman MS, Mascola JR, Wyatt RT, Wardemann H, Nussenzweig MC. A method for identification of HIV gp140 binding memory B cells in human blood. J Immunol Methods. 2008 Dec 25

Scheid JF, Hugo Mouquet, Niklas Feldhann, Michael S Seaman, Klara Velinzon, John Pietzsch, Rene Ott, Robert M Anthony, Henry Zebroski, Arlene Hurley, Adhuna Phogat, Bimal Chakrabarti, Yuxing Li, Mark Connors, Florencia Pereyra, Bruce D Walker, Hedda Wardemann, David Ho, Richard T Wyatt, John R Mascola, Jeffery V Ravetch and Michel C Nussenzweig. Broad diversity of neutralizing antibodies isolated from memory B cells in HIV infected individuals. Nature 2009, in press.

Barna Dey, Krisha Svehla, Ling Xu, Dianne Wycuff, Tongqing Zhou, Gerald Voss, Adhuna Phogat, Bimal K. Chakrabarti, Yuxing Li, George Shaw, Peter D.Kwong, Gary Nabel, John Mascola and Richard Wyatt. Structure-based Modification of HIV-1 gp120 Dramatically Enhances Conformational Stabilization and Host Immune Responses to a Discontinuous Epitop. In press, PLoS Pathogens 2009

Pia Dosenovic, Bimal K. Chakrabarti, Iyadh Douagi, Martina Soldemo, Mattias N. E. Forsell, Adhuna Phogat, Yuxing Li, Staffan Paulie, James Hoxie, Richard T. Wyatt and Gunilla B. Karlsson Hedestam. Use of an HIV-1 Env-specific B cell ELISpot assay for quantitative and qualitative analysis of Env-specific B cell responses following immunization. JImmunol 2009