Genomics Core

The Scripps Research Institute (TSRI) Genomics Core Facility provides cutting-edge genomic technologies, resources and solutions to faculties, investigators, and clinicians at TSRI. Our expertise includes Next-Generation Sequencing (NGS), NGS library creation, single-cell genomics, spatialomics, metagenomics, custom microarray fabrication and analysis. In addition, the Genomics Core Facility supports experimental design, grant application, interpretation of sequencing and microarray results and education of TSRI students, staff and faculty. We aim to stay aligned with the ever-evolving "omics" technologies for the scientific advancement at TSRI community and beyond.

About

Services

Instruments

Sequencing

  1. Element Aviti
  2. Illumina Nextseq200
  3. Oxford Nanopore Minion

 

Genomics Assays

  1. 10X Genomics Chromium X (single cell)
  2. NanoString (Bruker) GeoMX digital spatial profiler

 

RNA/DNA analysisAgilent Bioanalyzer 2100

  1. Agilent Tapestation
  2. Thermo Fisher Qubit
  3. Thermo Fisher Fluoroskan Microplate Fluorometer
  4. SpectraMax Mini Multi-Mode Microplate Reader

 

Microarray

  1. MicroGrid II contact microarray printer
  2. Innoscan 1100AL confocal microarray scanner

 

Liquid handling

  1. Opentrons (OT-2)
  2. Eppendorf epMotion 5075
  3. SPT Labtech Mosquito HV

 

PCR

  1. Bio-Rad QX200 digital droplet PCR

Publications

Sample Submission

Internal Users:

  • First-time internal users need to request access to the genomics core and submit a service request through ELAN INFINITY (Located in MyApps).
  • Please follow the instructions listed within the ELAN INFINITY Scripps Genomics Core Submission Portal. There will be a link within the portal for you to download the Genomics Core Project Submission Sheet
  • Use the Genomics Core Project Submission Sheet to provide the technical information for your project. Email completed form to sequencing@scripps.edu and your point of contact (POC) staff scientist.
  • Sample drop off:
    • Option 1: Bring your samples to the genomics core and hand off to the core staff;
    • Option 2: Leave your samples in the sample drop off freezer located outside of MB-28. To use the sample drop-off freezer option, your samples must be in a box/bag, clearly labeled with your name, PI name, and drop off date. Please take a picture of your samples in the freezer and email to John or Jessica.

 

External Users:

  • First-time external users need to contact Sheila at shkay@scripps.edu 858-784-2084 to set up an IDEA ELAN INFINITY account.
  • Please follow the instructions listed within the ELAN INFINITY Scripps Genomics Core Submission Portal. There will be a link within the portal for you to download the Genomics Core Project Submission Sheet
  • Use the Genomics Core Project Submission Sheet to provide the technical information for your project. Email completed form to dnaseq@scripps.edu and your point of contact (POC) staff scientist.
  • Please note that our shipping address is different than our laboratory physical address. Shipment using the incorrect address may delay the project timeline.

 

Shipping Address:

Attn: (list contact person at the Genomics Core Facility)
Genomics Core Facility
9060 Activity Rd Unit F
MB28
San Diego, CA 92126

Contact Us

Position Name Email Phone
Senior Director Dr. Sean Wang xiongwang@scripps.edu 858-784-2373
Senior Director (retiring) Steve Head shead@scripps.edu 858-784-2218
Project Manager Sheila Kay shkay@scripps.edu 858-784-2084
Senior Scientific Manager Tony Mondala tmondala@scripps.edu 858-784-2262
Scientific Associate Ryan McBride rmcbride@scripps.edu 858-784-2263
Research Assistant III John Shimashita jshimo@scripps.edu 858-784-2463
Research Assistant III Jessica Ledesma jledesma@scripps.edu 858-784-2467
Research Technician Joseph Koo jkoo@scripps.edu N/A
Administrative Assistant Nina Tung ninat@scripps.edu 858-784-2680
Genomics Core Main Line N/A N/A 858-784-2263