GUY'S 13

Oct 8, 2001

Hi Craig,

Here are preliminary TIFF files, they do not yet have labels. We hope to do that on Monday. The files are uncompressed, 1-2MB each.

guy13ca.tif - The Calpha backbone, labels for the 6 CDRs will be added. View is looking down into binding pocket.

guy13side.l.tif and guy13side.r.tif - Two views of the side chains making up the antigen-binding pocket. Together these 2 views create a stereo pair. When we add labels, I will try to get them into the same file. One is the left and one the right view. The right view is identical to the Calpha backbone figure. If you print them out small, you can glue them together to see the stereo. It was hard to get all the side chains in. Tyr L49 and Tyr L94 are omitted, as well as some of the CDR H2 residues, which are shown in the next figure. Side chains are shown extending from the Calpha backbone.

guy13h2.l.tif and guy13h2.r.tif - Two views of CDR H2 side chains (together a stereo pair). The antigen-binding pocket is rotated to the top. I picked this view to show that Tyrs H50 and H52 extend into the binding pocket, Ile H51 extends into the framework structure, and Pro H52A is part of the loop structure at the top of CDR H2, that also includes Tyr H53 and Asn H54. Side chains are shown extending from the Calpha backbone except around Pro H52A, where it is necessary to show the full backbone or the Pro looks weird.



Oct 10, 2001

Here are TIFF files with residue numbers for the current state of the Guy13 model. I used Kabat numbers and numbered CDR H3 the way you did in the text. If there is a mistake in the numbering, it can be fixed, let me know. The files are uncompressed, 1-4MB each.

guy13ca.lbl.tif - As above with CDRs labeled.

guy13side.l.lbl.tif and guy13side.r.lbl.tif - As above with all shown side chains labeled.

guy13h2.l.lbl.tif and guy13h2.r.lbl.tif - As above with most shown side chains labeled, H53 and H54 were too difficult to put in, but i think they are clear from the context since H50-H52A are labeled. Vickie

TIFF files

guy13ca.tif
guy13side.l.tif
guy13side.r.tif
guy13h2.l.tif
guy13h2.r.tif



Labeled TIFF files

guy13ca.lbl.tif
guy13side.l.lbl.tif
guy13side.r.lbl.tif
guy13h2.l.lbl.tif
guy13h2.r.lbl.tif