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Matthew Macauley, Ph.D. The Scripps Research Institute 10550 North Torrey Pines Road, MB-209 La Jolla, CA 92037 858-784-9668(phone) 858-784-9690 (FAX) e-mail: mmacaule@scripps.edu |
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EDUCATION: 2010 - Ph. D. Biochemistry, Simon Fraser University, Burnaby, British Columbia (Canada) 2003 - B.S. Biochemistry, University of British Columbia (Canada) PROFESSIONAL EXPERIENCES: October 2014 to Present - Assistant Professor of Chemical Physiology, The Scripps Research Institute, La Jolla, CA, USA June 2010 to September 2014 - Postdoctoral Fellow, The Scripps Research Institute, La Jolla, CA, USA (Advisor: Prof. James C. Paulson) September 2004 to May 2010 - Doctoral Candidate, Department of Chemistry @ Simon Fraser University, Burnaby, British Columbia, Canada (Advisor: Prof. David J. Vocadlo) May 2002 to September 2004 - Research Technician, Department of Biochemistry @ University of British Columbia, Canada (Supervisor: Dr. L.P. McIntosh) ACADEMIC AND PROFESSIONAL HONORS: 2010-2013: Human Frontier Science Program Organization (HFSPO) - Doctoral Fellowship 2011: The CCUCC (Canadian Council of University Chemistry Chairs) Doctoral Thesis Award 2011: AAI (American Association of Immunologists) - travel award 2008: Simon Fraser University Chemistry Poster Competition - poster prize 2006-2009: Natural Sciences and Engineering Research Council of Canada (NSERC) - Canadian Graduate Fellowship (PhD) 2007-2009: Michael Smith Foundation for Health Research - Senior Trainee Fellowship 2006: Merck Frosst Research Day - selected as a participant 2006: XXIIIrd International Carbohydrate Symposium - poster prize 2005-2006: Natural Sciences and Engineering Research Council of Canada (NSERC) - Canadian Graduate Fellowship (Masters) 2005: PENCE Annual Research Conference - poster prize 2005-2007: Michael Smith Foundation for Health Research - Junior Trainee Fellowship 2003: UBC Faculty of Medicine - Summer Research Scholarship 2002: UBC Undergraduate Research Conference - poster prize PUBLICATIONS: Pang L, Macauley MS, Arlian BM, Nycholat CM, Paulson JC. Encapsulating An Immunosuppressant Enhances Tolerance Induction by Siglec Engaging Tolerogenic Liposomes. Chembiochem. 2017;18(13):1226-1233. [PMID: 28231415] Lopez Aguilar A, Briard JG, Yang L, Ovryn B, Macauley MS, Wu P. Tools for Studying Glycans: Recent Advances in Chemoenzymatic Glycan Labeling. ACS Chem Biol. 2017 Mar 17;12(3):611-621. [PMID: 28301937] Orgel KA, Duan S, Wright BL, Maleki SJ, Wolf JC, Vickery BP, Burks AW, Paulson JC, Kulis MD, Macauley MS. Exploiting CD22 on antigen-specific B cells to prevent allergy to the major peanut allergen Ara h 2. J Allergy Clin Immunol. 2017 Jan;139(1):366-369.e2. [PMID: 27554819] Murinello S, Moreno SK, Macauley MS, Sakimoto S, Westenskow PD, Friedlander M. Assessing Retinal Microglial Phagocytic Function In Vivo Using a Flow Cytometry-based Assay. J Vis Exp. 2016;(116) [PMID: 27805590] Voss JE, Macauley MS, Rogers KA, Villinger F, Duan L, Shang L, Fink EA, Andrabi R, Colantonio AD, Robinson JE, Johnson RP, Burton DR, Haase AT. Reproducing SIV nef vaccine correlates of protection: trimeric gp41 antibody concentrated at mucosal front lines. AIDS. 2016:30(16):2427-2438. [PMID: 27428745] Gicheva N, Macauley MS, Arlian BM, Paulson JC, Kawasaki N. Siglec-F is a novel intestinal M cell marker. Biochem Biophys Res Commun. 2016;479(1):1-4. [PMID: 27524237] McCoy LE, van Gils MJ, Ozorowski G, Messmer T, Briney B, Voss JE, Kulp DW, Macauley MS, Sok D, Pauthner M, Menis S, Cottrell CA, Torres JL, Hsueh J, Schief WR, Wilson IA, Ward AB, Sanders RW, Burton DR.Holes in the Glycan Shield of the Native HIV Envelope Are a Target of Trimer-Elicited Neutralizing Antibodies. 2016;16(9):2327-38. [PMCID: PMC5007210] Natoni A, Macauley MS, O'Dwyer ME. Targeting Selectins and Their Ligands in Cancer. Front Oncol. 2016;6:93. [PMID: 27148485] Jin HY, Gonzalez-Martin A, Miletic AV, Lai M, Knight S, Sabouri-Ghomi M, Head SR, Macauley MS, Rickert RC, Xiao C. Transfection of microRNA Mimics Should Be Used with Caution. Front Genet. 2015;6:340. [PMID: 26697058] Macauley MS, Kawasaki N, Peng W, Wang SH, He Y, Arlian BM, McBride R, Kanagi R, Khoo KH, Paulson JC. Unmasking of CD22 on germinal center B-cells occurs by alternative mechanisms in mouse and man. J Biol Chem. 2015;290(50):30066-77. [PMID: 26507663] Angata T, Nycholat CM, Macauley MS. Therapeutic Targeting of Siglecs using Antibody- and Glycan-Based Approaches. Trends Pharmacol Sci. 2015;36(10):645-60. [PMID: 26435210] Macauley MS, Arlian BM, Rillahan CD, Pang PC, Bortell N, Marcondes MC, Haslam SM, Dell A, Paulson JC. Systemic blockade of sialylation in mice with a global inhibitor of sialyltransferases. J Biol Chem. 2014;289(51):35149-58. [PMID: 25368325] Macauley MS, Paulson JC. Siglecs Induce Tolerance to Cell Surface Antigens by BIM-Dependent Deletion of the Antigen-Reactive B Cells. J Immunol. 2014;193(9):4312-21. [PMID: 25252961] Macauley MS, Crocker PR, Paulson JC. Siglec-mediated regulation of immune cell function in disease. Nat Rev Immunol. 2014;14(10):653-66. [PMID: 25234143] Lee M, Kiefel H, LaJevic MD, Macauley MS, O'Hara E, Pan J, Paulson JC, Butcher EC. Transcriptional programs of lymphoid tissue capillary and high endothelium reveal control mechanisms for lymphocyte homing. Nat Immunol. 2014;15(10):982-95 [PMID: 25173345] Kawasaki N, Rillahan CD, Cheng TY, Van Rhijn I, Macauley MS, Moody DB, Paulson JC. Targeted Delivery of Mycobacterial Antigens to Human Dendritic Cells via Siglec-7 Induces Robust T Cell Activation. J Immunol. 2014;193(4):1560-1566. [PMID: 25000981] Rillahan CD, Macauley MS, Schwartz E, He Y, McBride R, Arlian BM, Rangarajan J, Fokin VV, Paulson JC. Disubstituted Sialic Acid Ligands Targeting Siglecs CD33 and CD22 Associated with Myeloid Leukaemias and B Cell Lymphomas. Chem Sci. 2014;5(6):2398-2406. [PMID: 24921038] Macauley MS, Paulson JC. Immunology: Glyco-engineering 'super-self'. Nat Chem Biol. 2013;10(1):7-8. [PMID: 24346034] Pfrengle F, Macauley MS, Kawasaki N, Paulson JC. Copresentation of Antigen and Ligands of Siglec-G Induces B Cell Tolerance Independent of CD22. J Immunol. 2013;191(4):1724-1731.[PMID: 23836061] Macauley MS, Pfrengle F, Rademacher C, Nycholat CM, Gale AJ, von Drygalski A, Paulson JC. Antigenic liposomes displaying CD22 ligands induce antigen-specific B cell apoptosis. J Clin Invest. 2013;123(7):3074-3083.[PMID: 23722906] Paulson JC, Macauley MS, Kawasaki N. Siglecs as sensors of self in innate and adaptive immune responses. Ann N Y Acad Sci. 2012;1253(1):37-48.[PMID: 22288608] Yuzwa SA, Shan X, Macauley MS, Clark T, Skorobogatko Y, Vosseller K, Vocadl DJ. Incraing O-GlcNAc slows neurodegeneration and stabilizes tau against aggregation. Nat Chem Biol. 2012;8(4):393-9. [PMID: 22366723] Macauley MS, Chan J, Zandberg WF, He Y, Whitworth GE, Stubbs KA, Yuzwa SA, Bennet AJ, Varki A, Davies GJ, Vocadlo DJ. Metabolism of vertebrate amino sugars with N-glycolyl groups: intracellular b-O-linked N-glycolylglucosamine (GlcNGc), UDP-GlcNGc, and the biochemical and structural rationale for the substrate tolerance of b-O-linked b-N-acetylglucosaminidase. J Biol Chem. 2012;287(34):28882-97. [PMID: 22692202] Macauley MS, Shan X, Yuzwa SA, Gloster TM, Vocadlo DJ. Elevation of Global O-GlcNAc in rodents using a selective O-GlcNAcase inhibitor does not cause insulin resistance or perturb glucohomeostasis. Chem Biol. 2010;17(9):949-58. [PMID: 20851344] Macauley MS, He Y, Gloster TM, Stubbs KA, Davies GJ, Vocadlo DJ. Inhibition of O-GlcNAcase using a potent and cell-permeable inhibitor does not induce insulin resistance in 3T3-L1 adipocytes. Chem Biol. 2010;17(9):937-48. [PMID: 20851343] Macauley MS, Vocadlo DJ. Increasing O-GlcNAc levels: An overview of small-molecule inhibitors of O-GlcNAcase. Biochim Biophys Acta. 2010;1800(2):107-21. [PMID: 19664691] He Y, Macauley MS, Stubbs KA, Vocadlo DJ, Davies GJ. Visualizing the reaction coordinate of an O-GlcNAc hydrolase. J Am Chem Soc. 2010;132(6):1807-9. [PMID: 20067256] Stubbs KA, Macauley MS, Vocadlo DJ. A selective inhibitor Gal-PUGNAc of human lysosomal beta-hexosaminidases modulates levels of the ganglioside GM2 in neuroblastoma cells. Angew Chem Int Ed Engl. 2009;48(7):1300-3. [PMID: 19130519] Sinclair DA, Syrzycka M, Macauley MS, Rastgardani T, Komljenovic I, Vocadlo DJ, Brock HW, Honda BM. Drosophila O-GlcNAc transferase (OGT) is encoded by the Polycomb group (PcG) gene, super sex combs (sxc). Proc Natl Acad Sci U S A. 2009;106(32):13427-32. [PMCID: PMC2726349] Macauley MS, Vocadlo DJ. Enzymatic characterization and inhibition of the nuclear variant of human O-GlcNAcase. Carbohydr Res. 2009;344(9):1079-84. [PMID: 19423084] Greig IR, Macauley MS, Williams IH, Vocadlo DJ. Probing synergy between two catalytic strategies in the glycoside hydrolase O-GlcNAcase using multiple linear free energy relationships. J Am Chem Soc. 2009;131(37):13415-22. [PMID: 19715310] Abbott DW, Macauley MS, Vocadlo DJ, Boraston AB. Streptococcus pneumoniae endohexosaminidase D, structural and mechanistic insight into substrate-assisted catalysis in family 85 glycoside hydrolases. J Biol Chem. 2009;284(17):11676-89. [PMCID: PMC2670171] Yuzwa SA, Macauley MS, Heinonen JE, Shan X, Dennis RJ, He Y, Whitworth GE, Stubbs KA, McEachern EJ, Davies GJ, Vocadlo DJ. A potent mechanism-inspired O-GlcNAcase inhibitor that blocks phosphorylation of tau in vivo. Nat Chem Biol. 2008;4(8):483-90. [PMID: 18587388] Martinez-Fleites C, Macauley MS, He Y, Shen DL, Vocadlo DJ, Davies GJ. Structure of an O-GlcNAc transferase homolog provides insight into intracellular glycosylation. Nat Struct Mol Biol. 2008;15(7):764-5. [PMID: 18536723] Macauley MS, Bubb AK, Martinez-Fleites C, Davies GJ, Vocadlo DJ. Elevation of global O-GlcNAc levels in 3T3-L1 adipocytes by selective inhibition of O-GlcNAcase does not induce insulin resistance. J Biol Chem. 2008;283(50):34687-95. [PMID: 18842583] Whitworth GE, Macauley MS, Stubbs KA, Dennis RJ, Taylor EJ, Davies GJ, Greig IR, Vocadlo DJ. Analysis of PUGNAc and NAG-thiazoline as transition state analogues for human O-GlcNAcase: mechanistic and structural insights into inhibitor selectivity and transition state poise. J Am Chem Soc. 2007;129(3):635-44. [PMID: 17227027] Stubbs KA, Macauley MS, Vocadlo DJ. A highly concise preparation of O-deacetylated arylthioglycosides of N-acetyl-D-glucosamine from 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-alpha-D-glucopyranosyl chloride and aryl thiols or disulfides. Carbohydr Res. 2006;341(10):1764-9. [PMID: 16472788] Sheldon WL, Macauley MS, Taylor EJ, Robinson CE, Charnock SJ, Davies GJ, Vocadlo DJ, Black GW. Functional analysis of a group A streptococcal glycoside hydrolase Spy1600 from family 84 reveals it is a beta-N-acetylglucosaminidase and not a hyaluronidase. Biochem J. 2006;399(2):241-7. [PMCID: PMC1609908] Macauley MS, Errington WJ, Scharpf M, Mackereth CD, Blaszczak AG, Graves BJ, McIntosh LP. Beads-on-a-string, characterization of ETS-1 sumoylated within its flexible N-terminal sequence. J Biol Chem. 2006;281(7):4164-72. [PMID: 16319071] Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ. Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity. Nat Struct Mol Biol. 2006;13(4):365-71. [PMID: 16565725] Cetinbas N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ. Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants. Biochemistry. 2006;45(11):3835-44. [PMID: 16533067] Macauley MS, Whitworth GE, Debowski AW, Chin D, Vocadlo DJ. O-GlcNAcase uses substrate-assisted catalysis: kinetic analysis and development of highly selective mechanism-inspired inhibitors. J Biol Chem. 2005;280(27):25313-22. [PMID: 15795231] Macauley MS, Stubbs KA, Vocadlo DJ. O-GlcNAcase catalyzes cleavage of thioglycosides without general acid catalysis. J Am Chem Soc. 2005;127(49):17202-3. [PMID: 16332065] Macauley MS, Errington WJ, Okon M, Scharpf M, Mackereth CD, Schulman BA, McIntosh LP. Structural and dynamic independence of isopeptide-linked RanGAP1 and SUMO-1. J Biol Chem. 2004;279(47):49131-7. [PMID: 15355965] PATENTS: Vocadlo, DJ, McEachern EJ, Macauley MS; Whitworth G, Heinonen J, Li T, Stubbs K. 'Selective glycosidase inhibitors and uses thereof' PCT Int. Appl. WO 2008025170, 2008 . Vocadlo DJ, Macauley MS, Whitworth G. 'Selective glycosidase inhibitors, methods of making inhibitors, and uses thereof' PCT Int. Appl. WO 2006092049, 2006. |
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