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Faculty Lecture Series
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Wednesday, November 10, 2010
5 PM - 6 PM |
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Valerie Timken Amphitheater
Green Hospital |
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Philip Dawson, Ph.D.
Associate Professor
Department of Chemistry
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Topic |
"Synthetic Protein Chemistry" |
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The ability to systematically modify proteins through site directed mutagenesis has transformed protein science, enabling structure-function studies to be performed in a routine manner. However, despite the utility of these methods, there is a growing demand for proteins that are modified in a manner incompatible with normal cellular ribosomal expression. Chemical protein synthesis is attractive for these studies since there are very few limitations on the structural perturbations that can be introduced, which include unnatural amino acid side chains, backbones, site-specific post-translational modifications and biophysical probes. Synthetically, proteins present unique challenges due to their large size and high density of functional groups. In order to avoid late stage deprotection and solubility problems, we have focused on the development of rapid, high yielding reactions that are fully compatible with unprotected peptides using water as the reaction media. The development and refinement of these so called chemical ligation reactions will be presented with a focus on how basic principles of chemical reactivity can be applied to complex molecular systems. |
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