Source: Interfolio F180
Shannon Miller, PhD
Scripps Research Joint Appointments
Research Focus
Genome editing holds promise as a one-time cure for genetic disease, but issues with toxicity, types of DNA manipulations, and biomolecule delivery limit therapeutic application. The Miller Lab develops and applies technologies for the in vivo use of gene editing tools for the safe and effective study and treatment of genetic disease. We tackle problems spanning biology and chemistry, with specific emphasis on protein engineering, directed evolution, viral and non-viral delivery, and genome editing. Our research is currently focusing on three major areas: (1) the development of novel AAV vectors for improved in vivo use and industrial production, (2) the development of a novel method for large DNA manipulations, and (3) improving in vivo CRISPR therapeutics using fully chemically modified sgRNAs.
Education
Ph.D. (Chemistry and Chemical Biology), Harvard University, 2022B.S. (Chemistry), University of Illinois Urbana-Champaign, 2016
Awards & Professional Activities
Forbes 30 under 30 North America Science, 2022
Keystone Symposia Scholarship, Engineering the Genome, 2020
Frontiers in Genome Engineering selected oral presentation, 2018
Bok Center Certificate of Distinction in Teaching, 2017
NSF Graduate Research Fellowship Program Fellowship, 2016-2019
RC Fuson Memorial Award for Distinction in Undergraduate Research, 2016
ACS Outstanding Senior Organic Chemistry Student Award, 2016
Lubrizol Foundation Scholarship, 2015
Dow Undergraduate Research Scholarship, 2015
James Scholar Preble Research Award, 2015
Peter C. and Gretchen Miller Markunas Scholarship, 2014
Dow Chemical Summer Undergraduate Research Fellowship, 2014
Lincoln Hall Excellence Scholar, Univeristy of Illinois Urbana-Champaign, 2012-2016
Selected Publications
Huang, Tony P.; Heins, Zachary J.; Miller, Shannon M.; Wong, Brandon G.; Balivada, P A.; Wang, Tina; Khalil, Ahmad S.; Liu, David R. High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs. 2023, 41, 96-107.
miller, shannon m.; liu, david r.; weiss, mitchell j.; tsai, shengdar q.; pruett-miller, shondra m.; kalfa, theodosia a.; tisdale, john f.; mccaffrey, anton p.; koblan, luke w.; wang, tina; zhao, kevin t.; richter, michelle; henderson, jordana m.; sharma, akshay; lechauve, christophe; thaman, elizabeth; podracky, christopher j.; jang, yoonjeong; everette, kelcee a.; mayberry, kalin; yao, yu; porter, shaina n.; tillman, heather; li, yichao; lazzarotto, cicera r.; mayuranathan, thiyagaraj; woodard, kaitly j.; yen, jonathan; newby, gregory a. Base editing of haematopoietic stem cells rescues sickle cell disease in mice. Nature 2021.
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miller, shannon m.; liu, david r.; wang, tina Phage-assisted continuous and non-continuous evolution. Nature Protocols 2020.
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miller, shannon m.; liu, david r.; rees, holly a.; newby, gregory a.; matuszek, zaneta; huang, tony p.; shen, max w.; arbab, mandana; randolph, peyton b.; wang, tina Continuous evolution of SpCas9 variants compatible with non-G PAMs. Nature Biotechnology 2020.
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miller, shannon m.; liu, david r.; savage, david f.; fellmann, christof; oakes, benjamin l.; gaudelli, nicole m.; zhao, kevin t.; huang, tony p. Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors. Nature Biotechnology 2019.
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miller, shannon m.; hennighausen, lothar; liu, chengyu; liu, david r.; smith, harold c.; willi, michaela; lee, hye k. Simultaneous targeting of linked loci in mouse embryos using base editing. Scientific Reports 2019.
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